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pcmv6 entry plasmid  (OriGene)


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    OriGene pcmv6 entry plasmid
    Pcmv6 Entry Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pcmv6+entry/pCMV6-Entry+Mammalian+Expression+Vector/pmc13217847-87-0-3
    Average 96 stars, based on 1672 article reviews
    pcmv6 entry plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA, number CRL-1721) were grown in RPMI 1640 supplemented with 10% heat-inactivated horse serum, 5% fetal bovine serum, and 50 μg/ml of gentamycin at 37 °C under 5% CO 2 . .. Expression vector pCMV6-Entry bearing human proBDNF cDNA ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001709","term_id":"1519314581","term_text":"NM_001709"}} NM_001709 , Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce His 6 affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi-Site-directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions and mutagenic primers: 5′-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3′ (sense) and 5′-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3′. ..

    Article Title: Nuclear Role of WASp in Gene Transcription is Uncoupled from its ARP2/3-Dependent Cytoplasmic Role in Actin Polymerization
    Article Snippet: .. Full-length WASp cDNA was subcloned into mammalian expression vector pCMV6-Entry containing Flag (also known as DDK) and Myc dual-tags at the C-terminal end (Origene). ..

    Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA # CRL-1721) were grown in RPMI-1640 supplemented with 10% heatinactivated horse serum, 5% fetal bovine serum (FBS) and 50 μg/ml gentamycin at 37°C under 5% CO2. by guest on N ovem ber 5, 2019 http://w w w .jbc.org/ D ow nloaded from .. Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’. ..

    Article Title: Nuclear Role of WASp in Gene Transcription is Uncoupled from its ARP2/3-Dependent Cytoplasmic Role in Actin Polymerization
    Article Snippet: .. WASp domain-deleted mutants Full-length WASp cDNA was subcloned into mammalian expression vector pCMV6-Entry containing Flag (also known as DDK) and Myc dual-tags at the C-terminal end (Origene). ..

    Article Title: BACE1 – but not BACE2 – function is critical for metabolic disorders induced by high-fat diets in C57BL/6N mice
    Article Snippet: .. The mouse Tmem27 gene (NM_020626) was expressed from the expression vector pCMV6- Entry, with a myc-FLAG epitope tag on the C-terminus (MR223526, Origene Technologies, Rockville, MD, USA). .. The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).

    Article Title: Effect of BTD gene variants on in vitro biotinidase activity.
    Article Snippet: Introduction: Biotinidase deficiency (BD), an autosomal recessive disease, is classified into profound (activity <10%) or partial BD (activity 10-30%).. The most frequent variant in patients worldwide is c.1330G>C (p.Asp444His), which is associated with partial BD.. In vivo studies indicate that this variant reduces the biotinidase activity by 50%.

    Modification:

    Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA, number CRL-1721) were grown in RPMI 1640 supplemented with 10% heat-inactivated horse serum, 5% fetal bovine serum, and 50 μg/ml of gentamycin at 37 °C under 5% CO 2 . .. Expression vector pCMV6-Entry bearing human proBDNF cDNA ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001709","term_id":"1519314581","term_text":"NM_001709"}} NM_001709 , Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce His 6 affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi-Site-directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions and mutagenic primers: 5′-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3′ (sense) and 5′-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3′. ..

    Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA # CRL-1721) were grown in RPMI-1640 supplemented with 10% heatinactivated horse serum, 5% fetal bovine serum (FBS) and 50 μg/ml gentamycin at 37°C under 5% CO2. by guest on N ovem ber 5, 2019 http://w w w .jbc.org/ D ow nloaded from .. Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’. ..

    Introduce:

    Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA, number CRL-1721) were grown in RPMI 1640 supplemented with 10% heat-inactivated horse serum, 5% fetal bovine serum, and 50 μg/ml of gentamycin at 37 °C under 5% CO 2 . .. Expression vector pCMV6-Entry bearing human proBDNF cDNA ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001709","term_id":"1519314581","term_text":"NM_001709"}} NM_001709 , Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce His 6 affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi-Site-directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions and mutagenic primers: 5′-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3′ (sense) and 5′-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3′. ..

    Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA # CRL-1721) were grown in RPMI-1640 supplemented with 10% heatinactivated horse serum, 5% fetal bovine serum (FBS) and 50 μg/ml gentamycin at 37°C under 5% CO2. by guest on N ovem ber 5, 2019 http://w w w .jbc.org/ D ow nloaded from .. Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’. ..

    Sequencing:

    Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA, number CRL-1721) were grown in RPMI 1640 supplemented with 10% heat-inactivated horse serum, 5% fetal bovine serum, and 50 μg/ml of gentamycin at 37 °C under 5% CO 2 . .. Expression vector pCMV6-Entry bearing human proBDNF cDNA ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001709","term_id":"1519314581","term_text":"NM_001709"}} NM_001709 , Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce His 6 affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi-Site-directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions and mutagenic primers: 5′-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3′ (sense) and 5′-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3′. ..

    Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA # CRL-1721) were grown in RPMI-1640 supplemented with 10% heatinactivated horse serum, 5% fetal bovine serum (FBS) and 50 μg/ml gentamycin at 37°C under 5% CO2. by guest on N ovem ber 5, 2019 http://w w w .jbc.org/ D ow nloaded from .. Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’. ..

    Mutagenesis:

    Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA, number CRL-1721) were grown in RPMI 1640 supplemented with 10% heat-inactivated horse serum, 5% fetal bovine serum, and 50 μg/ml of gentamycin at 37 °C under 5% CO 2 . .. Expression vector pCMV6-Entry bearing human proBDNF cDNA ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001709","term_id":"1519314581","term_text":"NM_001709"}} NM_001709 , Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce His 6 affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi-Site-directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions and mutagenic primers: 5′-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3′ (sense) and 5′-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3′. ..

    Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA # CRL-1721) were grown in RPMI-1640 supplemented with 10% heatinactivated horse serum, 5% fetal bovine serum (FBS) and 50 μg/ml gentamycin at 37°C under 5% CO2. by guest on N ovem ber 5, 2019 http://w w w .jbc.org/ D ow nloaded from .. Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’. ..

    Article Title: Effect of BTD gene variants on in vitro biotinidase activity.
    Article Snippet: Introduction: Biotinidase deficiency (BD), an autosomal recessive disease, is classified into profound (activity <10%) or partial BD (activity 10-30%).. The most frequent variant in patients worldwide is c.1330G>C (p.Asp444His), which is associated with partial BD.. In vivo studies indicate that this variant reduces the biotinidase activity by 50%.

    Countercurrent Chromatography:

    Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA, number CRL-1721) were grown in RPMI 1640 supplemented with 10% heat-inactivated horse serum, 5% fetal bovine serum, and 50 μg/ml of gentamycin at 37 °C under 5% CO 2 . .. Expression vector pCMV6-Entry bearing human proBDNF cDNA ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001709","term_id":"1519314581","term_text":"NM_001709"}} NM_001709 , Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce His 6 affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi-Site-directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions and mutagenic primers: 5′-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3′ (sense) and 5′-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3′. ..

    Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
    Article Snippet: Rat pheochromocytoma PC12 (ATCC, Manassas, VA # CRL-1721) were grown in RPMI-1640 supplemented with 10% heatinactivated horse serum, 5% fetal bovine serum (FBS) and 50 μg/ml gentamycin at 37°C under 5% CO2. by guest on N ovem ber 5, 2019 http://w w w .jbc.org/ D ow nloaded from .. Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’. ..

    Plasmid Preparation:

    Article Title: Nuclear Role of WASp in Gene Transcription is Uncoupled from its ARP2/3-Dependent Cytoplasmic Role in Actin Polymerization
    Article Snippet: .. Full-length WASp cDNA was subcloned into mammalian expression vector pCMV6-Entry containing Flag (also known as DDK) and Myc dual-tags at the C-terminal end (Origene). ..

    Article Title: Nuclear Role of WASp in Gene Transcription is Uncoupled from its ARP2/3-Dependent Cytoplasmic Role in Actin Polymerization
    Article Snippet: .. WASp domain-deleted mutants Full-length WASp cDNA was subcloned into mammalian expression vector pCMV6-Entry containing Flag (also known as DDK) and Myc dual-tags at the C-terminal end (Origene). ..

    Article Title: Effect of BTD gene variants on in vitro biotinidase activity.
    Article Snippet: Introduction: Biotinidase deficiency (BD), an autosomal recessive disease, is classified into profound (activity <10%) or partial BD (activity 10-30%).. The most frequent variant in patients worldwide is c.1330G>C (p.Asp444His), which is associated with partial BD.. In vivo studies indicate that this variant reduces the biotinidase activity by 50%.

    other:

    Article Title: Nitration and Glycation Turn Mature NGF into a Toxic Factor for Motor Neurons: A Role for p75 NTR and RAGE Signaling in ALS
    Article Snippet: Stable transfection was performed using Lipofectamine 2000 (Life Technologies), and the culture media were supplemented with 350 μg/mL G418 sulfate (Corning) and 200 μg/mL hygromycin B.

    Construct:

    Article Title: Effect of BTD gene variants on in vitro biotinidase activity.
    Article Snippet: Introduction: Biotinidase deficiency (BD), an autosomal recessive disease, is classified into profound (activity <10%) or partial BD (activity 10-30%).. The most frequent variant in patients worldwide is c.1330G>C (p.Asp444His), which is associated with partial BD.. In vivo studies indicate that this variant reduces the biotinidase activity by 50%.

    Gene Expression:

    Article Title: Effect of BTD gene variants on in vitro biotinidase activity.
    Article Snippet: Introduction: Biotinidase deficiency (BD), an autosomal recessive disease, is classified into profound (activity <10%) or partial BD (activity 10-30%).. The most frequent variant in patients worldwide is c.1330G>C (p.Asp444His), which is associated with partial BD.. In vivo studies indicate that this variant reduces the biotinidase activity by 50%.

    Cell Culture:

    Article Title: Effect of BTD gene variants on in vitro biotinidase activity.
    Article Snippet: Introduction: Biotinidase deficiency (BD), an autosomal recessive disease, is classified into profound (activity <10%) or partial BD (activity 10-30%).. The most frequent variant in patients worldwide is c.1330G>C (p.Asp444His), which is associated with partial BD.. In vivo studies indicate that this variant reduces the biotinidase activity by 50%.



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